Collaborators: David A. C. Beck (eScience & Chemical Engineering), Jisun Paik (Comparative Medicine), Lillian Maggio-Price (Comparative Medicine), Piper Treuting (Comparative Medicine), (Gastroenterology)
Inflammatory Bowel Disease (IBD) is associated with increased colon-cancer risk. It is not clear why ≈10% of chronic colitis patients get neoplastic progression, while the vast majority do not. The gut microbiome differs between healthy individuals and patients with IBD or colon cancer. We hypothesize that the microbiota play a role in the development of colitis-associated colon cancer (CAC). To address this, we will use the Smad3-/- mouse model of CAC, where approximately 40% of mice develop cancer after gut inflammation is triggered by bacteria. We previously noted that the gut microbiota of mice that develop cancer (progressors) differs from that of cancer-free mice (non-progressors). We will expand on these findings by testing Koch’s postulate: eg. by transferring fecal samples of mice with and without cancer into germ-free Smad3-/- mice and examining for cancer development. Next, we will evaluate the translational potential of these mice as a bio-indicator/avatars of CAC in humans. We will transfer mucosa-associated microbiota of IBD patients (progressors vs. non-progressors) into germ-free Smad3-/- mice and examine for neoplastic development. Our studies will address the causative role of microbiota on CAC, develop a potential bioassay for assessing CAC risk, and identify microbial targets to modulate incidence of CAC.
- Potential for using a hermetically-sealed, positive-pressured isocage system for studies involving germ-free mice outside a flexible-film isolator.